Identification of differentially expressed genes in rheumatoid arthritis by a combination of complementary DNA array and RNA arbitrarily primed-polymerase chain reaction

The first adult-repopulating hematopoietic stem cells (HSCs) emerge in the mouse aorta-gonad-mesonephros (AGM) region at embryonic day 10.5 prior to their appearance in the yolk sac and fetal liver. Although several genes are implicated in the regulation of HSCs, there are gaps in our understanding of the processes taking place in the AGM at the time of HSC emergence. To identify genes involved in AGM HSC emergence, we performed differential display reverse transcriptase–polymerase chain reaction (DD RT-PCR). Differentially expressed genes included β-catenin and homologs of human TM9SF2 and TAB2. We characterized the expression pattern of Wnt/β-catenin signaling,mTM9SF2, and mTAB2 in the embryo and adult. Interestingly, the expression of mouse TAB2 (mTAB2) in the E11 dorsal aorta endothelium suggests a role for mTAB2 in HSC emergence and/or regulation. The identification of differentially expressed genes in the AGM region should yield further insights into the development of this tissue and into the emergence and regulation of HSCs.

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Direct DNA Sequencing of Complementary DNA Amplified by the Polymerase Chain Reaction

Protocols in Human Molecular Genetics ◽

10.1385/0-89603-205-1:9 ◽

2003 ◽

pp. 9-20

Author(s):

Richard A. Gibbs ◽

Phi-Nga Nguyen ◽

C. Thomas Caskey

Keyword(s):

Polymerase Chain Reaction ◽

Dna Sequencing ◽

Chain Reaction ◽

Complementary Dna ◽

Direct Dna Sequencing ◽

Polymerase Chain

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Annexin IV is Differentially Expressed in Clear Cell Carcinoma of the Ovary

International Journal of Gynecological Cancer ◽

10.1111/igc.0b013e3181a844c8 ◽

2009 ◽

Vol 19 (9) ◽

pp. 1545-1549 ◽

Cited By ~ 20

Author(s):

Yi Miao ◽

Bin Cai ◽

Ling Liu ◽

Yixia Yang ◽

Xiaoping Wan

Keyword(s):

Cell Carcinoma ◽

Dna Microarray ◽

Clear Cell ◽

Clear Cell Carcinoma ◽

Differentially Expressed ◽

Serous Carcinoma ◽

Chain Reaction ◽

Complementary Dna ◽

Polymerase Chain ◽

Annexin Iv

Objective:To investigate the genes that were differentially expressed between clear cell carcinoma (CCC) and serous carcinoma (SAC) of the ovary with complementary DNA microarray.Methods:Complementary DNA microarray was carried out in 8 CCCs and 8 SACs of the ovary. Differentially expressed genes were identified and verified by real-time polymerase chain reaction. The expression of the protein was also verified with immunohistochemistry and Western blot in cells and tissues of ovarian CCC.Results:Comparison of the gene expression profiling identified 21 genes with more than 2-fold different expression between CCC and SAC of the ovary. The up-regulated and down-regulated genes were 9 and 12, respectively. The verification of Annexin IV in the cell line and tissues was in accordance with the result of the microarray.Conclusions:The complementary DNA microarray technique is a feasible way to explore the difference of the gene expression profiling between the 2 types of ovarian carcinoma. The overexpression of Annexin IV may be an ovarian CCC-specific molecular marker.Abbreviations:CCC- clear cell carcinoma, SAC- serous carcinoma, PCR- polymerase chain reaction, RT-PCR- reverse transcriptase PCR, ABCF2- ATP-binding cassette, sub-family F- member 2, HNF-1b- hepatocyte nuclear factor 1β

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